i am doing my thesis on bsf substrate. I wanna study about the storage performance and larval performance of various substrates. mainly 4 different samples are tested. one is the control feed, one wi

i am doing my thesis on bsf substrate. I wanna study about the storage performance and larval performance of various substrates. mainly 4 different samples are tested. one is the control feed, one with lab Plantarum, yeast and a combination of lab and yeast. this is a 4 week experiment. so initialy the substrate is shredded to 5 mm size. ten its heated to 70 C and kept at that temperature for 1 hr as a standard practise as required by the EU regulation. the substrate is made up of supermarket waste. however no meat or fish is added as per the EU regulation. the substrate contains 10 percent bread, 10 percent egg, 10 percent yogurt and 70 percent vegetables and fruits. (31.82 percent banana, 25.45 apple, 4.24 leafy vegetables, 3.39 capsicum, 3.39 zucchini, 1.69 carrot . ) . all of this is W/W. Before the substrate is heated 2 percent v/v straw is added to the mixture . this is because the subtrate would be so wet and there would be poor aeration and structural stability otherwise when stored. now after the subtrate is heated and cooled down we add the required organisms to it and the samples are stored in 100ml bottles. now sample sare taken in duplicates. so there are in total 40 samples. this is because we are storing the larvae for day 1, 7,14,21 and 28. now lwts see how each sample are made. control is directly stored after the subtrate is cooled down. yeast sample is made by adding yeats to the control sample 24 hrs before its fed to the larvae. for examplefor day 7 , we add yeast on day 6 and keep it at 30C for 24 hrs aerobically and feed it to larvae. all samples are stored anerobically. the containers are filled completely so that there is no air space. this is true for all samples. for lab , the sample is innoculated with lab 24 hrs before storage unlike yeast. for eg for all the lab samples on day 0 the substrate is innoculated with lab and then stored at 30C for 24hrs anerobically and then moved to room conditions. and on the proposed day larvae are inroduced. for lab plus yeast also known as the Z sample hereafter we inoculate it with lab before storage and then aerobically add yeast 24hrs befor eintroduction of larvae. so for day 1 weadd lab on day -1 and then yeast on day 0 and add larvae on day 1. now every week larvaes are introduced we use larve 5 days old. the sample fro the 100 ml container which has 104g of control subtrate before any kinds of treatment is introduced to a 1 L container. 70 larvaes are added to each container and straw of 3g to absorb additional moisture. then these larvae are kept at 30C for 14 days and harvested. can you help me write a 60 page thesis. ignore result and conclusion and references.
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