I cultured microglia from disease mouse model and run mitostress seahorse with oligomycin, fccp and rotenone with antimycin a. All the respiration parameters are increased. However ATP production meas
I cultured microglia from disease mouse model and run mitostress seahorse with oligomycin, fccp and rotenone with antimycin a. All the respiration parameters are increased. However ATP production measured by a separate assay shows no difference. Can you give me some possible reasons?
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